Journal: Nature Communications

Article Title: Thrombopoiesis is spatially regulated by the bone marrow vasculature

doi: 10.1038/s41467-017-00201-7

Figure Lengend Snippet: MKs are largely sessile and distributed within the BM. Distribution of megakaryocytes (MKs, CD41 + , green ) in cryo-sections of femur a and sternum b showing vessels (CD105 + , red ) and nuclei (Hoechst33258, blue , Scale bar: 1 mm ( left panels ), 100 µm ( right panels ). c MKs were counter-stained for β1-tubulin ( magenta , indicated with arrowheads —in contrast to β1 − MKs which are indicated with arrows ); scale bar 20 µm. These mature MKs were larger d , but distributed similarly as depicted by the percentage of vessel-associated MKs e and the distance to vessels of non-vessel associated (NVA) MKs f . g The percentage of β1-tubulin positive CD41-cells within 100 µm distance from the bone cortex (BA bone-associated) and more distant MKs (NBA non-bone associated) was similar. Bar graphs represent mean ± SD; n = 4. ** p < 0.01. There are no significant differences in e – g ( p > 0.05; Mann–Whitney U test)

Article Snippet: Nuclei were stained with Hoechst33258 (Biomol, Hamburg, Germany) and mounted with Mowiol (Sigma-Aldrich) or 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI)-containing mounting medium (DAPI Fluoromount-G; Southern Biotech, Birmingham, AL, USA).

Techniques: Staining, MANN-WHITNEY